Separation and identification of anthocyanin from rice husk of oryza sativa l. cv. Chainat 1

Abstract

In this work, separation and identification of anthocyanin from rice husk of Oryza sativa L. cv. Chainat1 was carried out. The combination of dichloromethane and ethyl acetate crude extracts was isolated by various chromatographic techniques. The isolation and the structure determination of these pink compound was performed as described previously. Compound 2 was indicated that it is one type of anthocyanin. Malvidin-3-rutinoside is a major pigment that give pink color in this compound. Malvidin-3-rutinoside and BHT showed the IC₅₀ value of 145 ± 30 and 11.6 ± 2.6 μg/ml, respectively.