Chemical constituents and their biological activity of stem barks of Croton oblongifolius Roxb. from Amphoe Muang, Uttaradit province

Abstract

In the investigation of chemical constituents of the stem barks of Croton oblongifolius Roxb., 2 new diterpenoid compounds, (2E, 7E, 11E)-1-isopropyl-1,4-dihydroxy-4,8-dimethylcyclotetradeca-2,7,11-triene-12-carboxylic acid, Methyl-15,16-epoxy-12-oxo-3,13 (16),14-clerodatriene-20,19-olide-17-oate and 4 known diterpenoids, (-)-Pimara-9(11),15-diene-19-oic acid, Crotocembraneic acid, Neocrotocembraneic acid, (-)-Pimara-9(11),15-diene-19-ol were isolated. The structure of these compounds were established by spectroscopic data such as IR, MS spectra, 1D and 2D techniques including DEPT, COSY, NOESY, HMBC and HMQC. All of compounds were tested biological activity against a panel of six cell lines including fibroblast (HS27), gastric carcinoma (KATO-3), breast carcinoma (BT474), lung carcinoma (CHAGO), colon carcinoma (SW620), hepato carcinoma (HEP-G2) (-)-Pimara-9(11),15-diene-19-ol showed cytoxic activity against the gastric carcinoma (KaTO-3, lung carcinoma (CHAGO), colon carcinoma (SW620) and hepato carcinoma (HEP-G2), in vitro, with IC50 values of 6.5, 6.1, 5.9 and 6.7 mu g/ml, respectively