SYNTHESIS AND ANTIBACTERIAL ACTIVITIES OF AMINO-CONTAINING CELLULOSIC MATERIALS

Abstract

Biofilm formation and bacteria adhesion on surfaces are major problems for both public health and several industries. To solve these problems, considerable attention has been directed toward developing contacted-based bactericidal surfaces. In this research, series of amino-containing molecules including 3-aminopropyltrimethoxysilane (APS), polyethyleneimine (PEI), 1,4-diazabicyclo[2.2.2] octane (DABCO), and 4,7,10-trioxa-1,13-tridecanediamine (DA) were immobilized on cotton surfaces via various chemical reactions. The physical properties of these surfaces were determined using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-ray Photoelectron Spectroscopy (XPS), and Scanning Electron Microscopy (SEM). Primary and quaternary amine loading were quantified using CI acid orange 7 and fluorescein sodium salt, respectively. Antibacterial activities of all surfaces were evaluated using colony counting method (JIS L 1902) in both Gram-positive (Staphylococcus aureus (ATCC 6538P) and Gram-negative bacteria (Escherichia coli (ATCC 8739) and Pseudomonas aeruginosa (ATCC 9027)). DABCO-C16 with permanent positive charges and Phys.PEI with the abilities to be protonated showed broad-spectrum antibacterial activities with complete killing. Although APS and DA did not show complete killing, they were still considered highly active with around 5 log CFU reduction. Moreover, cytotoxicity assays of modified cottons toward L-929 mouse connective tissue fibroblast were conducted. The results confirmed that DABCO and DA and its derivative were biocompatible with mammalian cells.