Effects of genistein on oxidative stress and inflammation in acute pancreatitis mice

Abstract

Genistein (GEN), the most abundant isoflavones in soy, has similar structure to human estradiol. It has been reported the potent properties of GEN on antioxidant and anti-inflammation. The aim of this study was to investigate the effects of GEN on oxidative stress, inflammatory, and histopathology in acute pancreatitis induced by L-arginine in mice. Male ICR mice were randomly divided into 4 groups (6 mice in each group). Control (Con) group: mice were injected with 2% dimethyl sulfoxide (DMSO) once daily for 4 days, via IP. Acute pancreatitis (AP) group: mice were injected 2 times (1 hour interval) of 350 mg/100 g body weight L-arginine which dissolved in 0.9% normal saline to induce acute pancreatitis. AP + low dose genistein (LG) group: mice were received 10 mg/kg GEN, while AP + high dose GEN (HG) group: mice were received 100 mg/kg GEN, dissolved in 2% DMSO administered by IP injection 2 hours before the induction of L-arginine, then daily injection of GEN for 3 days. Body weight change was compared between at the beginning and the end of study. Serum and pancreas were collected 72 hours after induction of L- arg. The result showed that, low and high doses of genistein were able to attenuate severity of acute pancreatitis which characterized by restored body weight loss, decreased serum AMY, inflammatory cytokines (IL-6, CPR, and MPO), pancreatic MDA, and decreased histological damage. In the present study increased understanding of the effects of genistein on oxidative stress, inflammation as well as pancreatic histopathology in L-arg induced acute pancreatitis mice.