SYNTHESIS OF PUERARIN-GOLD NANOPARTICLES FOR TRANSDERMAL DELIVERY

Abstract

Puerarin (Pur) is herbal medicine that its inflammatory response but low solubility to reduce activity. Thus, the aim of this study was to synthesize q188-kappa-carrageenan@Pur@gold nanoparticles (q188-κ-c@Pur@AuNPs) for enhancing solubility and transdermal delivery. The κ-c was synthesized by quaternization of κ-c with 25% (v/v) q188, and the q188-κ-c @AuNPs was prepared by reducing HAuCl4 into AuNPs by using 25% (v/v) q188-κ-c as reducing and stabilizing agents. The highest degree of quaternization (%DQ) at 21.12% was found q188 addition, which was calculated from 1H NMR. The solubility of the q188-κ-c derivatives was 43.7±0.01%, which higher than κ-c. The size of synthesized q188-κ-c@AuNPs was confirmed by UV-vis, TEM, EDX and DLS, which were found the successfully synthesized with the size 15 nm. A cytotoxic study of Pur loaded on q188-κ-c-@AuNPs derivatives (q188-κ-c-@Pur@AuNPs) were observed, which shown increasing cytotoxicity to CLS cells and non-toxic to normal cells (Wi-38) by MTT assay. In vitro drug permeation was studied by Franz’s cell method. The q188-κ-c-@Pur@AuNPs permeation was investigates by ICP-OES and UV-vis that shown increasing permeation to across cellulose membrane. According to the results, it was suggested that q188-κ-c@Pur@AuNPs can be a good candidate to further development for transdermal delivery applications.