Qualitative analysis of essential oils using TLC stationary phase modification

Abstract

Agarwood essential oil has a unique aroma and is well known in the Arab countries due to its value and price. Due to its high price, it has been developed into various grades. Basically, in order to investigate the adulterants and characteristics of authentic agarwood oil, gas chromatography or gas chromatography-mass spectrometry are used to classify its volatile compounds which is slightly complicated and time consuming. However, thin-layer chromatography (TLC) was modified in stationary phase with silver nitrate to increase the polarity of the stationary phase to successfully classify the unsaturated complex compounds of agarwood oils. This report aims to find the most optimum procedure under suitable conditions which are 0.5% AgNo₃ (w/v) with 80:20 ratio (methanol:milli-Q), then immersed the TLC plate in solution for 10 minutes and took in the oven at 110°C for 15 minutes. To separate the same genus from different procedures. Gas chromatography-mass spectrometry was shown that silver nitrate gave a difference in the compound selectivity. Lastly, Color intensity was to identify as a potential application as can identify a fingerprint of further essential oils.