Mitochondrial DNA sequence variation within and between red junglefowl (Gallus gallus gallus and Gallus gallus spadiceus) in Thailand

Abstract

Two subspecies of red junglefowl are described using the difference in their earlobe color. Gallus gallus gallus carries a white earlobe while that of G.g.spadiceus is red. 10 animals from each subspecies were measured for 5 morphomtetric parameters. The analysis found that 4 out of 5 showed nonsignificant differences gut one character, the beak length, showed significance difference between two subspecies using the analysis of variance (ANOVA). DNA sequence analysis of 225 base pairs in the mitochondrial control region (D-loop) showed phylogenetic branching of the two subspecies using genetic distance analysis. Sequence divergence within the subspecies ranged from 0 to 6.54%, while the divergence between the two subspecies ranged from 1.34% to 8.0%.