Development of electrospun adhesive layer containing ciprofloxacin/coconut oil for antibacterial wound dressing

Abstract

Poly (vinyl acetate), a non-toxic adhesive, was used to prepare electro spun fibrous membranes by blending an antibiotic and wound healing supporter to cover commercial polyurethane (PU) substrate. PU film has many advantages such as excellent air permeation and good mechanical properties. This study focused on the synergistic interaction between coconut oil and Ciprofloxacin (CPF), which can be toxic to the growth of cells. The surface morphology, drug release were characterized by scanning electron microscopy (SEM) and UV-Vis spectroscopy. The antibacterial activity was tested against pathogenic bacteria, Escherichia coli and Staphylococcus aureus which are the representatives of gram (-) and gram (+) bacteria, respectively. The inhibition zones of E. coli and S.aureus appeared when CPF and coconut oil was loaded at 1.25 mg/mL and 0.5 mL, respectively. For the cytotoxicity of cell viability, the mats were evaluated with mouse fibroblast (L929) and human fibroblast cells by culturing on the wound dressing surface to determine indirect cytotoxicity using MTT essay and then the testing of mats showed good results for cell viability of both L929 and human fibroblast cells.