Lipid bilayer membrane penetration and antibody delivery of oxidized carbon nanoparticles

Abstract

Oxidized carbon nanoparticles (OCNs) were synthesized by oxidizing carbon black via modified Hummers and Offeman method. In this research, OCNs were used for delivering macromolecules into cells. The human monoclonal antibodies (HuMAbs) are an interesting macromolecules that were delivered into cells by the using of OCNs as a carrier. Because HuMAbs are synthesized antibodies from hybridoma technology that can neutralize all four serotypes of dengue viruses (DENV), as the cause of dengue fever. Nevertheless, HuMAbs cannot automatically enter cells so OCNs were essentially used to deliver HuMAbs into cells for intracellular DENV neutralization. OCNs can bring HuMAbs into Vero cells and the delivered HuMAbs still showed DENV neutralization activity. Moreover, the using of OCNs increase the neutralization ability of HuMAbs because it make HuMAbs can neutralize both intracellular and extracellular DENV. In addition, observation in our research group demonstrate that OCNs respond to heat. We hypothesized that NIR irradiation may accelerate the OCNs penetration rate into lipid bilayer membrane. We prepared the cell-sized liposome as lipid bilayer membrane by dehydration method. But, the using of OCNs with NIR irradiation cannot accelerate the OCNs penetration rate into the cell-sized liposome.