Isolation, screening, and cellulase assay of cellulolytic bacteria from rice field soils in Changwat Kalasin

Abstract

This study is focused on the isolation and screening the cellulolytic bacteria and assess the potential of bioaugmentation using isolated cellulolytic bacteria. The rice field soil from Kalasin province collected and screened for the active cellulolytic bacteria by Culturing technique. From the experiment, 40 of 60 isolates were defined as a cellulolytic bacterium by the Gram’s iodine staining test. The Hydrolysis Capacity (HC) estimation and statistical analysis showed that 10 isolates had a highest HC value and selected for cellulase assay and biodegradation efficiency test. They were named SB 13, SB 19, SB 22, SB 24 SB 29, SB 33, SB 39, SB 50, SB 52 and SB 59, respectively. Then the degradation efficiency test and morphological study confirmed that the SB 24 isolate is bacteria and showed the highest HC value. This can be concluded that active cellulolytic bacteria can be isolated from the rice field. And due to the Covid-19 situation, the laboratory was closed, cellulose assays were not conducted and the efficacy of degradation was tested.