Hydrocarbon Degradation Potentials of Some Bacteria Species Isolated from Contaminated Soil with Spent Lubricating Oil.

Abstract

This research aims to select bacteria that can biodegrading of used lubricating, measures carbon dioxide from bacterial activity in the degradation of lubricants, and study morphology of bacteria that isolated from contaminated soil with lubricants and collect the example of soil from a garage in Chiang Mai. This research found that could be isolated 4 strains of bacteria by take 10 g of sample soil and dissolve in distilled water 90 ml. Then, take 10 ml in culture medium BHM 100 ml (BHM + lubricating oil 1% + Tween 20 1%) and shake 100 rpm at 37 degrees for 24-72 hr. repeat 3 times. After that, take the bacteria dilute from 10⁻¹-10⁻⁷, and spread it on the surface of Trypticase Soy Agar or TSA by Spread Plate Technique. Then, incubate at 37 degrees for 24-48 hr. After that, selecting bacteria for purification by Streaking Plate Technique and incubate at 37 degrees for 24 hr. Then, gram staining all 4 bacteria. This research found that all of them are gram negative bacteria, entire (smooth), convex, curve, and opaque.